During Desert Shield and Desert Storm, USMC’s 86 AV-8Bs flew over 3,380 missions and 4,100 hours with a mission availability of over 90 percent.Short answer is yes. At sea, one and one-third squadrons of AV-8Bs supported the 4th and 5th Marine Expeditionary Brigades from USS Nassau (LHA-4) and USS Tarawa (LHA-1).
Source is Osprey Aircraft book AV-8B HARRIER II UNITS OF OPERATIONS DESERT SHIELD AND DESERT STORM. PDF is too large to attach.
Five were lost to enemy SAM's with two fatalities. It had an attrition rate of 1.5 aircraft for every 1,000 sorties flown. U.S. Army General Schwarzkopf nominated it as one of the seven weapons that had played a crucial role in the war.